Now, medical examiners have determined the cause of death of Ms Vallow’s brother Alex Cox, 51, who was found dead on the floor of his bathroom in December.
Mr Cox died of a blood clot in his lung and heart disease, according to the Maricopa County Medical Examiner’s Office in Arizona.
In a statement, Mr Cox’s family said the coroner’s report proved his death “had no sinister aspect”.
“With a degree of vindication, the family hopes that the confirmation of this truth will help all to step back, take a breath, and allow the truth to unfold through the process,” the family’s attorney, Robert P. Jarvis said.
“Rampant rumours of evil plots and conspiracy robbed the family of their time to grieve the loss of their husband, son, brother, and uncle.
“Even so, the family finds comfort in the medical examiner’s finding that Alex Cox died of natural causes.”
In July 2019, Mr Cox fatally shot his sister’s estranged husband, Charles Vallow, which he described as self-defence. Police said there was a physical confrontation between Mr Cox and Mr Vallow and no arrests were made.
Mr Cox’s sister, Ms Vallow, and her current husband, Chad Daybell, are also under investigation for murder, attempted murder and conspiracy in the death of Mr Daybell’s former wife, Tammy.
The 49-year-old was found dead in her home in October with her death initially being ruled as natural. But in December, her body was exhumed so an autopsy could be carried out. The results are still pending.
Ms Vallow and Mr Daybell married just weeks after Tammy’s death but no charged have been laid in that case.
Police have previously said Tammy’s death may be linked to the disappearance of Ms Vallow’s children.
Ms Vallow is being held on a US$1 million bond and has pleaded not guilty to the charges.
Her husband – a self-published author who has written more than two dozen books about near-death and doomsday events – has not been charged in connection to the children’s disappearance.
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